The correct answer is 'Does'. The full sentence should read: 'Does he speak English?' This is a question in the present simple tense, and because the subject 'he' is third-person singular, we need the auxiliary verb 'does'. Therefore, the correct option is 'Does'.
In English, we use 'does' for questions with 'he,' 'she,' or 'it' in the simple present tense, while we use 'do' for 'I,' 'you,' 'we,' and 'they'. Since the question is about whether 'he' speaks English, we must use 'does' to form the question correctly.
So, the answer is:
'Does'